公车上拨开丁字裤进入电影,久久99精品久久久久久无毒不卡,国产丰满老熟妇乱XXX1区,夜夜爽妓女8888视频免费观看

當(dāng)前位置:首頁  >  技術(shù)文章  >  TROAP在STAT3的幫助下促進(jìn)腎透明細(xì)胞癌的增殖、遷移和轉(zhuǎn)移

TROAP在STAT3的幫助下促進(jìn)腎透明細(xì)胞癌的增殖、遷移和轉(zhuǎn)移

更新時(shí)間:2024-12-29  |  點(diǎn)擊率:140

20236月,江南大學(xué)附屬醫(yī)院泌尿外科;南京醫(yī)科大學(xué)第一附屬醫(yī)院泌尿外科;江南大學(xué)無錫醫(yī)學(xué)院 (Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi 214122, China;Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210008, China;Wuxi Medical College, Jiangnan University, Wuxi 214122, China) Jun Wang老師研究團(tuán)隊(duì)在《International Journal of Molecular Sciences》上發(fā)表論文:

 TROAP Promotes the Proliferation, Migration, and Metastasis of Kidney Renal Clear Cell Carcinoma with the Help of STAT3"

 

TROAPSTAT3的幫助下促進(jìn)腎透明細(xì)胞癌的增殖、遷移和轉(zhuǎn)移"

 

Abstract

Kidney renal clear cell carcinoma (KIRC) is a subtype of renal cell carcinoma that threatens human health. The mechanism by which the trophinin-associated protein (TROAP)-an important oncogenic factor-functions in KIRC has not been studied. This study investigated the specific mechanism by which TROAP functions in KIRC. TROAP expression in KIRC was analyzed using the RNAseq dataset from the Cancer Genome Atlas (TCGA) online database. The Mann-Whitney U test was used to analyze the expression of this gene from clinical data. The Kaplan-Meier method was used for the survival analysis of KIRC. The expression level of TROAP mRNA in the cells was detected using qRT-PCR. The proliferation, migration, apoptosis, and cell cycle of KIRC were detected using Celigo, MTT, wound healing, cell invasion assay, and flow cytometry. A mouse subcutaneous xenograft experiment was designed to demonstrate the effect of TROAP expression on KIRC growth in vivo. To further investigate the regulatory mechanism of TROAP, we performed co-immunoprecipitation (CO-IP) and shotgun liquid chromatography-tandem mass spectrometry (LC-MS). TCGA-related bioinformatics analysis showed that TROAP was significantly overexpressed in KIRC tissues and was related to higher T and pathological stages, and a poor prognosis. The inhibition of TROAP expression significantly reduced the proliferation of KIRC, affected the cell cycle, promoted cell apoptosis, and reduced cell migration and invasion. The subcutaneous xenograft experiments showed that the size and weight of the tumors in mice were significantly reduced after TROAP-knockdown. CO-IP and post-mass spectrometry bioinformatics analyses revealed that TROAP may combine with signal transducer and activator of transcription 3 (STAT3) to achieve tumor progression in KIRC; this was verified by functional recovery experiments. TROAP may regulate KIRC proliferation, migration, and metastasis by binding to STAT3.


摘要:

腎透明細(xì)胞癌(KIRC)是一種嚴(yán)重威脅人類健康的腎細(xì)胞癌亞型。TROAP是一種重要的致癌因子,其在KIRC中的作用機(jī)制尚未得到研究。本研究探討了TROAPKIRC中的具體作用機(jī)制。使用來自癌癥基因組圖譜(TCGA)在線數(shù)據(jù)庫的RNAseq數(shù)據(jù)集分析TROAPKIRC中的表達(dá)。采用Mann-Whitney U檢驗(yàn)從臨床資料中分析該基因的表達(dá)。采用Kaplan-Meier法進(jìn)行KIRC的生存分析。采用qRT-PCR檢測細(xì)胞中TROAP mRNA的表達(dá)水平。采用Celigo、MTT、創(chuàng)面愈合、細(xì)胞侵襲試驗(yàn)和流式細(xì)胞術(shù)檢測KIRC的增殖、遷移、凋亡和細(xì)胞周期。研究人員設(shè)計(jì)了小鼠皮下異種移植實(shí)驗(yàn)來證明TROAP表達(dá)對(duì)KIRC體內(nèi)生長的影響。為了進(jìn)一步研究TROAP的調(diào)控機(jī)制,研究人員采用了共免疫沉淀(CO-IP)和霰彈槍液相色譜-串聯(lián)質(zhì)譜(LC-MS)tcga相關(guān)生物信息學(xué)分析顯示,TROAPKIRC組織中顯著過表達(dá),與高T及病理分期、預(yù)后差有關(guān)。抑制TROAP表達(dá)可顯著降低KIRC的增殖,影響細(xì)胞周期,促進(jìn)細(xì)胞凋亡,減少細(xì)胞遷移和侵襲。皮下異種移植實(shí)驗(yàn)表明,敲除troap后,小鼠腫瘤的大小和重量明顯減小。CO-IP和質(zhì)譜后生物信息學(xué)分析顯示,TROAP可能與信號(hào)換能器和轉(zhuǎn)錄激活因子3 (STAT3)結(jié)合,在KIRC中實(shí)現(xiàn)腫瘤進(jìn)展;功能恢復(fù)實(shí)驗(yàn)證實(shí)了這一點(diǎn)。TROAP可能通過結(jié)合STAT3調(diào)控KIRC的增殖、遷移和轉(zhuǎn)移。

 

該論文中,對(duì)人KIRC細(xì)胞系786-O、ACHNCaki-1的體外培養(yǎng)是使用Ausbian特級(jí)胎牛血清完成的。


宝贝∽好硬∽好爽一再来| 客厅玩朋友娇妻HD完整版视频| 无码亚洲成A∧人片在线播放| 国产精品久久久久精品三级| 99久久久无码国产精品免费| 亚洲无码一区| 丰满少妇大乳高潮在线| 国产a√精品区二区三区四区| 99久久精品日本一区二区免费| 岳的大肥屁熟妇五十路99| 啊灬啊灬啊灬快灬深用力| 成人女人爽到高潮的A片| 狠狠做深爱婷婷久久综合一区| 亚洲 自拍 精品 在线 主播| 欧美AV噜噜狠狠网址蜜桃| 18禁裸乳无遮挡啪啪无码免费| 老女人重囗味456| 70岁老太把腿岔开给老头摸| A片在线免费观看| 无码人妻丰满熟妇区96| 国产麻豆精品一区二区三区V视界| 色婷婷国产精品视频一区二区三区 | 嫩模被强到高潮呻吟不断| 欧美变态深喉囗交XXXX| 久久这里只有精品| 孰妇XXXXXX的性生话| 中文乱码人妻系列一区二区| 中国xxxxxxxxx18| 欧洲熟妇色XXXX欧美老妇多毛 | 亚洲熟妇AV一区二区三区浪潮| 樱桃视频大全免费高清版 | 亚州AV综合色区无码一区| 精东影视传媒MV国产剧能看不| 老熟妇高潮一区二区三区| 特大欧美黑人巨大xxoo| 人妻丰满熟妞AV无码区| 蜜臀AV性久久久久蜜臀AⅤ麻豆| JAPANESE熟女俱乐部| 日本免费无码一区二区到五区| 成人午夜精品无码区久久 | 无码国产精品一区二区高潮|