公车上拨开丁字裤进入电影,久久99精品久久久久久无毒不卡,国产丰满老熟妇乱XXX1区,夜夜爽妓女8888视频免费观看

當(dāng)前位置:首頁  >  技術(shù)文章  >  TROAP在STAT3的幫助下促進(jìn)腎透明細(xì)胞癌的增殖、遷移和轉(zhuǎn)移

TROAP在STAT3的幫助下促進(jìn)腎透明細(xì)胞癌的增殖、遷移和轉(zhuǎn)移

更新時(shí)間:2024-12-29  |  點(diǎn)擊率:140

20236月,江南大學(xué)附屬醫(yī)院泌尿外科;南京醫(yī)科大學(xué)第一附屬醫(yī)院泌尿外科;江南大學(xué)無錫醫(yī)學(xué)院 (Department of Urology, Affiliated Hospital of Jiangnan University, Wuxi 214122, China;Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210008, China;Wuxi Medical College, Jiangnan University, Wuxi 214122, China) Jun Wang老師研究團(tuán)隊(duì)在《International Journal of Molecular Sciences》上發(fā)表論文:

 TROAP Promotes the Proliferation, Migration, and Metastasis of Kidney Renal Clear Cell Carcinoma with the Help of STAT3"

 

TROAPSTAT3的幫助下促進(jìn)腎透明細(xì)胞癌的增殖、遷移和轉(zhuǎn)移"

 

Abstract

Kidney renal clear cell carcinoma (KIRC) is a subtype of renal cell carcinoma that threatens human health. The mechanism by which the trophinin-associated protein (TROAP)-an important oncogenic factor-functions in KIRC has not been studied. This study investigated the specific mechanism by which TROAP functions in KIRC. TROAP expression in KIRC was analyzed using the RNAseq dataset from the Cancer Genome Atlas (TCGA) online database. The Mann-Whitney U test was used to analyze the expression of this gene from clinical data. The Kaplan-Meier method was used for the survival analysis of KIRC. The expression level of TROAP mRNA in the cells was detected using qRT-PCR. The proliferation, migration, apoptosis, and cell cycle of KIRC were detected using Celigo, MTT, wound healing, cell invasion assay, and flow cytometry. A mouse subcutaneous xenograft experiment was designed to demonstrate the effect of TROAP expression on KIRC growth in vivo. To further investigate the regulatory mechanism of TROAP, we performed co-immunoprecipitation (CO-IP) and shotgun liquid chromatography-tandem mass spectrometry (LC-MS). TCGA-related bioinformatics analysis showed that TROAP was significantly overexpressed in KIRC tissues and was related to higher T and pathological stages, and a poor prognosis. The inhibition of TROAP expression significantly reduced the proliferation of KIRC, affected the cell cycle, promoted cell apoptosis, and reduced cell migration and invasion. The subcutaneous xenograft experiments showed that the size and weight of the tumors in mice were significantly reduced after TROAP-knockdown. CO-IP and post-mass spectrometry bioinformatics analyses revealed that TROAP may combine with signal transducer and activator of transcription 3 (STAT3) to achieve tumor progression in KIRC; this was verified by functional recovery experiments. TROAP may regulate KIRC proliferation, migration, and metastasis by binding to STAT3.


摘要:

腎透明細(xì)胞癌(KIRC)是一種嚴(yán)重威脅人類健康的腎細(xì)胞癌亞型。TROAP是一種重要的致癌因子,其在KIRC中的作用機(jī)制尚未得到研究。本研究探討了TROAPKIRC中的具體作用機(jī)制。使用來自癌癥基因組圖譜(TCGA)在線數(shù)據(jù)庫的RNAseq數(shù)據(jù)集分析TROAPKIRC中的表達(dá)。采用Mann-Whitney U檢驗(yàn)從臨床資料中分析該基因的表達(dá)。采用Kaplan-Meier法進(jìn)行KIRC的生存分析。采用qRT-PCR檢測細(xì)胞中TROAP mRNA的表達(dá)水平。采用Celigo、MTT、創(chuàng)面愈合、細(xì)胞侵襲試驗(yàn)和流式細(xì)胞術(shù)檢測KIRC的增殖、遷移、凋亡和細(xì)胞周期。研究人員設(shè)計(jì)了小鼠皮下異種移植實(shí)驗(yàn)來證明TROAP表達(dá)對(duì)KIRC體內(nèi)生長的影響。為了進(jìn)一步研究TROAP的調(diào)控機(jī)制,研究人員采用了共免疫沉淀(CO-IP)和霰彈槍液相色譜-串聯(lián)質(zhì)譜(LC-MS)tcga相關(guān)生物信息學(xué)分析顯示,TROAPKIRC組織中顯著過表達(dá),與高T及病理分期、預(yù)后差有關(guān)。抑制TROAP表達(dá)可顯著降低KIRC的增殖,影響細(xì)胞周期,促進(jìn)細(xì)胞凋亡,減少細(xì)胞遷移和侵襲。皮下異種移植實(shí)驗(yàn)表明,敲除troap后,小鼠腫瘤的大小和重量明顯減小。CO-IP和質(zhì)譜后生物信息學(xué)分析顯示,TROAP可能與信號(hào)換能器和轉(zhuǎn)錄激活因子3 (STAT3)結(jié)合,在KIRC中實(shí)現(xiàn)腫瘤進(jìn)展;功能恢復(fù)實(shí)驗(yàn)證實(shí)了這一點(diǎn)。TROAP可能通過結(jié)合STAT3調(diào)控KIRC的增殖、遷移和轉(zhuǎn)移。

 

該論文中,對(duì)人KIRC細(xì)胞系786-O、ACHNCaki-1的體外培養(yǎng)是使用Ausbian特級(jí)胎牛血清完成的。


AAA级久久久精品无码片| 寡妇两腿间黑黑的毛毛是什么| 国内精品久久久人妻中文字幕| 无码精品a∨在线观看十八禁| 一夲道无码人妻精品一区二区| 国产无套粉嫩白浆在线观看| 诱人的女同学hd中文字幕| 日本护士被黑人强伦姧人妻孕| 欧美啪啪| 男人一边吃奶一边做爰免费视频| 国产女爽爽精品视频天美传媒| 视频一区二区三区sm重味| 日本A片把舌头伸进粉嫩| 日本不卡三区| 久久伊人蜜桃AV一区二区| 综影视之被各种男人啪H| 猛烈H继攵禁忌H| 成人性生交大片免费看视频| 亚洲精品v天堂中文字幕| 办公室少妇激情呻吟a片在线观看 无码又爽又刺激A片涩涩动漫 | 国产亚洲精品久久久久久久软件| 亚洲AV无码久久久久久精品同性| 国产韩国日本欧美的品牌SUV| 苍井空与黑人90分钟AV| 色之久久综合av人妻熟女黑寡妇| 久久无码人妻一区二区三区| 粗大的内捧猛烈进出小视频| 免费看撕开奶罩揉吮奶头视频| 精品久久久久久久免费人妻| 苍井空与黑人90分钟AV| 当着夫的面被夫上司玩弄| 老翁的大肉蟒进进出出| 51国产黑色丝袜高跟鞋| 上司人妻互换中文字幕| 白石茉莉奈AV| 18禁裸体动漫美女无遮挡网站| 无码av一区二区三区| 农村熟妇高潮精品A片| 久久人人爽天天玩人人妻精品| 妓女精品国产噜噜亚洲AV| 掀开奶罩边躁狠狠躁视频0000|